Scientific paper 31 – An experimental chemically inactivated HIV-1 vaccine induces antibodies that neutralize homologous and heterologous viruses

March 2, 2017 3:29 pm

An experimental chemically inactivated HIV-1 vaccine induces antibodies that neutralize homologous and heterologous viruses

Journal: Vaccine, Volume 13, Issue 1, January 1995, Pages 54-60

Authors: Esther Race, Pino Frezza,  D. Michael Stephens, David Davis, Natasha Polyanskaya, Martin Cranage, John S Oxford

Abstract

We have developed a unique multiple step procedure to inactivate human immunodeficiency virus chemically with a very high safety margin while retaining antigenically active structural virion proteins, including gp120, in the final immunogen.

The whole virus preparation (1–10 μg per dose) was highly immunogenic in a variety of small mammals and induced antibodies that recognized homologous and heterologous strains of HIV-1. Sera from immunized animals bound to peptides representing the entire sequence of the external glycoprotein gp120. Neutralizing antibodies active against the homologous immunizing strain and against heterologous HIV-1 strains were also elicited. Sera with virus neutralizing activity did not bind to MHC class I proteins derived from the human cell line used to grow the virus.

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