Scientific paper 32 – Initial in vitro screening of drug candidates for their potential antiviral activities

March 2, 2017 3:35 pm

Initial in vitro screening of drug candidates for their potential antiviral activities

Journal: Virology Methods Manual, Chapter · December 1996

Authors: Ali Al Jabri, M D Wigg, J S Oxford

Abstract

This chapter describes the in vitro methods used for screening antiviral agents against human immunodeficiency virus using cell suspension, herpes simplex, and influenza viruses cultivated in monolayer cell lines.
In addition, the chapter outlines an assay for virucidal activity. Candidate drugs are synthesized easily and cheaply, have long storage lives, and are active at micromolar levels. Preclinical evaluation of candidate agents for their antiviral activity in vitro with respect to both antiviral efficacy and cellular toxicity is the initial aim for antiviral drug development. In all biological assays, the exact concentration of an antiviral required to inhibit the replication of a virus depends on the infectivity input of the virus, the cell type used, the duration of the experiment, the kinetic of infection in that particular cell type, and the sensitivity of the assay being used.

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